Francesca Costantini

Work Experience

January 2014: Postdoctoral researcher at the Department of Astronautic, Electrical and Energetics engineering, Sapienza University (Rome, Italy). The research is focused on the development of novel surface chemistry techniques and their integration into lab-on- chip systems for the detection of biomarkers for the diagnosis of celiac disease and metabolic disorders such as diabetes and hypercholesterolemia. Detection of mycotoxins in food and beverage. Development of PCR on-chip and SELEX on-chip

December 2010–December 2013: Postdoctoral researcher at the Department of Chemistry. The research was focused on the development of novel nanomaterials based on polymer brushes for applications in the field of health-care and food quality control.

August 2009 – December 2010: Chemist at the Department of Bioimaging PET-CT at Cattolica University (Rome, Italy), for the synthesis of radiopharmaceuticals for PET applications, quality control and validation of analytical methods.

Education and Training

February 2005-June 2009: PhD student at Supramolecular Chemistry and Technology (SMCT) and Molecular Nanofabrication (MnF) groups (Http://mnf.tnw.utwente.nl, Enschede, The Netherlands), under the supervision of Prof. D.N. Reinhoudt and Jurriaan Huskens. The PhD research was focused on the immobilization of organic, metallic catalysts and enzymes on the surface interior of microchannels to study (bio)chemical reactions in sub-micrometer scale. Thesis Title: “Supported organic, NanoMetallic and Enzymatic catalysis in Microreactors”

July 2004-December 2004: Internship at Pepscam System B.V. (www.pepscan.nl Lelystad, The Netherlands). The Research was focused on the synthesis of organic compounds for medicinal chemistry.

November 2003-May 2004: Internship at Pharmacy “Al Sacro Cuore” (Bologna Italy) for attending the qualification exam for the pharmacist profession.

November 2003-May 2004: Internship at the Biochemistry Department of Bologna University (Bologna, Italy). The research was focused on studying the isomerization of mono/ polyunsaturated residues in phospholipids of cellular membranes (in vivo and in vitro) as a novel path of membrane damage.

September 1996-October 2003: Master Degree in Medicinal Chemistry, University of Bologna (Bologna, Italy): During the undergraduate period the research was focused on fatty acid chemistry. Master Thesis Title: "cis-trans isomers of unsaturated fatty acids in rat tissues" (Department of Biochemistry, Bologna, Italy).

Technical Skills and Competences: Biochemistry, organic synthesis, surface chemistry, polymer chemistry, radiochemistry and microfluidics. Gas Chromatography (GC), High Pressure Liquid Chromatography (HPLC), Spectrofluorimetry, Fluorescence Microscopy, HPLC-ES-MS, UV-Vis, FT-IR, 1H-NMR spectroscopy.

Languages: Italian: Mather-tongue, English: Excellent Dutch: Base

Computer Skills and Competences: Software: Microsoft Word and Excel, ChemDraw, CorelDraw, Power Point, SigmaPlot, KaleidaGraph, Origin.

Conferences:

9th Internationa Conference on Microreactor Technology (IMRET 9), September 6th -8th 2006, Postdam/ Berlin, Germany.

MicroTas 2007, October 7th -11th 2007, Paris, France.

Microfluidics 2008, 1st European Conference on Microfliudic (MicroFlu’2008) December 10th -12th 2008, Bologna, Italy.

3rd National congress of RadioPharmaceutical Chemistry, 30-31 October 2009, Pavia, Italy.

Nanoforum 2011, 13-15 September 2011, Rome, Italy. Convegno Giovani Chimici, 12-13 Giugno 2012, Rome, Italy. Nanoforum 2012, 24-26 September 2012, Rome, Italy. Nanomedicine, October 1-3 2012, Rome, Italy.

MicroTas 2013, October 27-31 2013, Freiburg, Germany.

Bio-Sensing Technology 2015, 10-13 May, Lisbon, Portugal.

Biosensors 2016, 25-27 May 2016, Goteborg, Sweden.

Scientific Contributions to Conferences:

“Catalytic Nanostructure screening in a Microfluidic Device“ MESA+ Symposium, September 2007 Enschede, The Netherlands, (Oral Communication).

“Nanostructure Based on Polymer Brushes for Heterogeneous Catalysis in Microreactors” MESA+ Symposium, September 2008 Enschede, The Netherlands, (Oral Communication).

“Polymer Brushes for Catalysts Immobilization on the Microchannel Inner Walls” NWO Meeting October 20th-22th 2008, Lunteren, The Netherlands, (Oral Communication).

“Nanostructures Based on Polymer Brushes for Heterogeneous Catalysis in Microreactors” MicroNano Conference (www.micronanoconference.nl) November 17th-19th 2008, Ede, The Netherlands (Oral Communication).

Polymer Brushes as New Stationary Phase for Ultra Thin Layer Chromatography, Convegno Giovani Chimici, 12th-13th June 2012, Rome, Italy, (Oral Communication).

Development of Lab-on-a-Chip Platforms for Point of Care Testing, Nanoforum 2012, Rome, Italy (Oral Communication).

Glucose level determination with enzymatic glass chip, Nanomedicine 2012, Rome, Italy (Oral Communication).

Self-assembling of Raman active silver nanoparticle-based microclusters exhibit versatile high-performance surface enhanced Raman spectroscopy: a combined high spatial resolution Raman spectroscopy and AFM approach. Plasmonica 2013, Milano (Italy) (Poster presentation).

Detection of ochratoxin a in food samples by a novel aptamer based sensor assay integrated in a microfluidic chip, Bioanalitica 2015, Firenze, Italy. (oral communication)

Design of a SELEX-chip for aptamers selection, Biosensors 2016, 25-27 May, Göteborg, Sweden (oral communication).

Publications:

Francesca Costantini, Wojchiech P. Bula, Riccardo Salvio, Jurriaan Huskens, Han J.G.E Gardeniers, David N. Reinhoudt and Willem Verboom “Nanostructure Based on Polymer Brushes for Efficient Heterogeneous Catalysis in Microreactors”, Journal of the American Chemical Society, 2009, 1650-1651.

Dirk Burdinski, Jeroen A. Pikkemaat, Mustafa Emrullahoglu, Francesca Costantini, Willem Verboom, Sander Langereis, Holger Grüll, and Jurriaan Huskens, “Targeted LipoCEST Contrast Agents for Magnetic Resonance Imaging: Alignment of Aspherical Liposomes on a Capillary Surface” Angewndte Chemie International Edition, 2010, 49, 2227 –2229.

Francesca Costantini, Edmondo M. Benetti, R. M. Tiggelaar, Han J.G.E Gardeniers, David N. Reinhoudt, Jurriaan Huskens, G.Julius Vancso and Willem Verboom “A Brush-Gel/Metal Nanoparticle Hybrid Film as an Efficient Supported Catalyst in Glass Microreactors” Chemistry-a European Journal, 2010, 16, 12406-12410.

Francesca Costantini, Edmondo M. Benetti, David N. Reinhoudt, Jurriaan Huskens , G. Julius Vancso and Willem Verboom “Enzyme-functionalized polymer brush films on the inner wall of silicon-glass microreactors with tunable biocatalytic activity” Lab on a Chip, 2010, 10, 3407-2412.

Cesare Manetti, Andrea Leonelli, Francesca Costantini Lab-on-chip system at “La Sapienza”: When Thin and Thick Film Technologies meet Chemistry and Biological Applications. Proceedings of a C.I.S.B. Minisymposium – June 2010.

Francesca Costantini, Fabio Domenici, Francesco Mura, Riccardo Scipinotti, Simona Sennato, Cesare Manetti, and Federico Bordi “A New Nanostructured Stationary Phase for Ultra-Thin Layer Chromatography: A Brush-Gel Polymer Film”, Nanoscience and Nanotechnology Letters, 2013, 5, 1155-1163.

Francesca Costantini, Roald Tiggelaar, Simona Sennato, Francesco Mura, Stefan Schlautmann, Federico Bordi, Han Gardeniers and Cesare Manetti “ Glucose level determination with a multi-enzymatic cascade reaction in a functionalized glass chip”, Analyst, 2013, 138, 5019–5024.

Francesca Costantini, Augusto Nascetti, Riccardo Scipinotti, Fabio Domenici, Simona Sennato, Laura Gazza, Federico Bordi, Norberto Pogna, Cesare Manetti, Domenico Caputo and Giampiero de Cesare “On-chip detection of multiple serum antibodies against epitopes of celiac disease by an array of amorphous silicon sensors” RSC Advances, 2014, 4, 2073-2080.

Domenico Caputo, Giampiero de Cesare, Riccardo Scipinotti, Nicolino Stasio, Francesca Costantini, Cesare Manetti and Augusto Nascetti “On-Chip Diagnosis of Celiac Disease by an Amorphous Silicon Chemiluminescence Detector” Sensors and Mycrosystems Lecture Notes in Electrical Engineering, 2014, 268, 183-187.

C. Fasolato F. Domenici, S. Sennato F. Mura, L. De Angelis, F. Luongo, F. Costantini, F. Bordi and P. Postorino, “Dimensional scale effects on Surface Enhanced Raman Scattering efficiency of self-assembled silver nanoparticle clusters” Applied Physics Letters 08/2014; 105(7):073105-073105-4.

Francesca Costantini, Augusto Nascetti, Giulia Petrucci, Cristiana Sberna, Cesare Manetti, Domenico Caputo and Giampiero de Cesare “Microfluidic chips with integrated amorphous silicon sensors for point-of-care testing”, 2014, 2053- 2055, Microtas 2014.

Francesca Costantini, Domenico Caputo, Giulia Petrucci, Giampiero de Cesare, Cristiana Sberna, Cesare Manetti, Augusto Nascetti, “Lab-on-chip system combining a microfluidic-ELISA with an array of amorphous silicon photosensors for the detection of celiac disease epitopes” Sensing and Bio- Sensing Research, 6, 51–58, 2015.

Francesca Costantini, Domenico Caputo, Giulia Petrucci, Giampiero de Cesare, Cristiana Sberna, Cesare Manetti, Augusto Nascetti, “Aptamer-based sandwich assay for on chip detection of Ochratoxin A by an array of amorphous silicon photosensors” Sensors and Actuators B: Chemical, 230, 31–39, 2016.

G. Petrucci, D. Caputo, , N. Lovecchio, F. Costantini, I. Legnini, I. Bozzoni, A. Nascetti, G. de Cesare, “Multifunctional System-on-Glass for Lab-on-Chip applications”, Biosensors & Bioelectronics, doi:10.1016/j.bios.2016.08.060, 2016.